Homoptera
Adult Suborder Homoptera Immature
CRDF | RNAi challenge
This work is a collaboration between the University of Arizona (Brown and Soderlund), Washington State University (Gang), and the University of Florida (Stansly and Roberts).

Funding: The Citrus Advanced Technology Program: The citrus psyllid transcriptome and time course differential gene expression in Ca. Liberibacter-infected/free whole psyllids and organs.

Asian Citrus Psyllid and Potato Psyllid Illumina Sequencing

Individual and comparison databases were created for the following datasets (ref #1, #2).
SpeciesLibraries
Asian Citrus Psyllid (ACP)   
Diaphorina citri
Whole-body adult, Whole-body adult CLas-infected,
Nymph, Nymph CLas-infected 
Potato Psyllid (PoP)
Bactericera cockerelli 
Whole-body adult, Whole-body adult CLso-infected,
Nymph, Nymph CLso-infected

- download the published transcriptomes or view the database using the TCW applet (ref #3).
 

Asian Citrus Psyllid Sanger and 454 Sequencing

Individual databases were created for Sanger ESTs (ref#4) and 454.
TypeLibraries
Sanger   ACP testes, ACP midgut, psyllid, Kuwayama  
454Whole body adult, gut

- download 454 reads and view the databases using the PAVE web interface (ref#5).
 

References

  1. TW Fisher, M Vyas, R He, W Nelson, JM Cicero, M Willer, R Kim, R Kramer, GA May, JA Crow, CA Soderlund, DR Gang and JK Brown (2014) Comparison of Potato and Asian Citrus Psyllid Adult and Nymph Transcriptomes Identified Vector Transcripts with Potential Involvement in Circulative, Propagative Liberibacter Transmission. Pathogens 3(4), 875-907; doi:10.3390/pathogens3040875. Link
  2. M Vyas, TW Fisher, R He, W Nelson, G Yin, JM Cicero, M Willer, R Kim, R Kramer, GA May, JA Crow, CA Soderlund, DR Gang, JK Brown (2015) Asian Citrus Psyllid Expression Profiles Suggest Candidatus Liberibacter Asiaticus-Mediated Alteration of Adult Nutrition and Metabolism, and of Nymphal Development and Immunity. PLOS One 10.1371/journal.pone.0130328 Link
  3. C Soderlund, W Nelson, M Willer, DR Gang (2013) TCW: Transcriptome Computational Workbench. PLOS One DOI:10.1371/journal.pone.0069401 Link
  4. Hunter et al. (2009) Psyllid Biology: Expressed Genes in Adult Asian Citrus Psyllids, Diaphorina citri Kuwayama The Open Entolmology Journal 3, 19-29. PDF
  5. Soderlund et al. (2009) PAVE: Program for Assembling and Viewing ESTs. BMC Genomics 10:400. Link

Email: www@agcol.arizona.edu